Ground test experiment for large space structures

In recent years a new body of control theory has been developed for the design of control systems for Large Space Structures (LSS). The problems of testing this theory on LSS hardware are aggravated by the expense and risk of actual in orbit tests. Ground tests on large space structures can provide a proving ground for candidate control systems, but such tests require a unique facility for their execution. The current development of such a facility at the NASA Marshall Space Flight Center (MSFC) is the subject of this report

NASA/MSFC ground experiment for large space structure control verification

Marshall Space Flight Center has developed a facility in which closed loop control of Large Space Structures (LSS) can be demonstrated and verified. The main objective of the facility is to verify LSS control system techniques so that on orbit performance can be ensured. The facility consists of an LSS test article which is connected to a payload mounting system that provides control torque commands. It is attached to a base excitation system which will simulate disturbances most likely to occur for Orbiter and DOD payloads. A control computer will contain the calibration software, the reference system, the alignment procedures, the telemetry software, and the control algorithms. The total system will be suspended in such a fashion that LSS test article has the characteristics common to all LSS

Definition of ground test for Large Space Structure (LSS) control verification

An overview for the definition of a ground test for the verification of Large Space Structure (LSS) control is given. The definition contains information on the description of the LSS ground verification experiment, the project management scheme, the design, development, fabrication and checkout of the subsystems, the systems engineering and integration, the hardware subsystems, the software, and a summary which includes future LSS ground test plans. Upon completion of these items, NASA/Marshall Space Flight Center will have an LSS ground test facility which will provide sufficient data on dynamics and control verification of LSS so that LSS flight system operations can be reasonably ensured

Definition of ground test for verification of large space structure control

Control theory and design, dynamic system modelling, and simulation of test scenarios are the main ideas discussed. The overall effort is the achievement at Marshall Space Flight Center of a successful ground test experiment of a large space structure. A simplified planar model of ground test experiment of a large space structure. A simplified planar model of ground test verification was developed. The elimination from that model of the uncontrollable rigid body modes was also examined. Also studied was the hardware/software of computation speed

Crystal Structure of PrgI-SipD: Insight into a Secretion Competent State of the Type Three Secretion System Needle Tip and its Interaction with Host Ligands

Many infectious Gram-negative bacteria, including Salmonella typhimurium, require a Type Three Secretion System (T3SS) to translocate virulence factors into host cells. The T3SS consists of a membrane protein complex and an extracellular needle together that form a continuous channel. Regulated secretion of virulence factors requires the presence of SipD at the T3SS needle tip in S. typhimurium. Here we report three-dimensional structures of individual SipD, SipD in fusion with the needle subunit PrgI, and of SipD:PrgI in complex with the bile salt, deoxycholate. Assembly of the complex involves major conformational changes in both SipD and PrgI. This rearrangement is mediated via a π bulge in the central SipD helix and is stabilized by conserved amino acids that may allow for specificity in the assembly and composition of the tip proteins. Five copies each of the needle subunit PrgI and SipD form the T3SS needle tip complex. Using surface plasmon resonance spectroscopy and crystal structure analysis we found that the T3SS needle tip complex binds deoxycholate with micromolar affinity via a cleft formed at the SipD:PrgI interface. In the structure-based three-dimensional model of the T3SS needle tip, the bound deoxycholate faces the host membrane. Recently, binding of SipD with bile salts present in the gut was shown to impede bacterial infection. Binding of bile salts to the SipD:PrgI interface in this particular arrangement may thus inhibit the T3SS function. The structures presented in this study provide insight into the open state of the T3SS needle tip. Our findings present the atomic details of the T3SS arrangement occurring at the pathogen-host interface